RESULTS AND DISCUSSION
Protoplasts of two cultivars of lettuce, Evola and Red Leaf Amboni green
fused products via PEG were cultured on four types of media show response
in T4 in all replications (Fig A-F) by repairing cell wall, division and
multiplication of cells in media (Fig: E-J), while remaining treatments show no
response to protoplast culture and burst immediately after culture in each
replication. Protoplast cultured in T4 gave highest number of micro-colonies
(Fig. K-N). Maximum micro colonies (73) were noted in R1 while minimum
micro-colonies (13) were observed in R5.