Work involving the standardization of the tetrazolium test methodology on other species of Rubiaceae can be found, focusing on reducing the time to detect the quality of seed lots. However, there has been different results according to the species studied. Fava and Albuquerque (2013), for example, have shown that the best procedure to estimate the viability of Palicourea rigida Kunth seeds was by using the tetrazolium solution at the concentration of 0.5% at 40 ºC for 3 hours.