The blocks were polished until a mirror-like surface is obtained and the ideal flatness of the cut face block allows observing the internal structure of starch by AFM. Fig. 1 A shows some example AFM topography images at low-resolution of starch granules from ripe bananas. Although this cultivar is composed by granules with different sizes(7 to 50 m) and shapes (round and elongated), depending on the stage of ripening (Peroni-Okita et al., 2010), it is likely that the shapes and sizes observed in Fig. 1 A might be due to the orientation of granules within the resin as not all the cuts occur through the center of every granule.