Bioassays. The extract was applied in a dose of 50 g/l in order to determine the biological efficacy of the plant extracts on the mortality of T. urticae adults, as well as their effect on the inhibition of oviposition in surviving adults. These were applied
to bean plants (Phaseolus vulgaris L. cv. Aidagold), which were planted in a regular garden substrate, in pots of 10-cm diameter. At the time the experiment was being established, 2–3 leaves were found in the growth phase (the growing top of the plants
was removed before application to make sure that further plant growth did not affect the experiment).
The day before application, a binocular magnifier and a fine brush were used to introduce 10 females of T. urticae (1–2 days old) onto each of the leaves.
The extract was applied to the plants using a manual electronic atomiser in a dose approximately equivalent to the application of 600 l water/ha. Control plants were treated using only water. The experiment was repeated 5 times. The plants were placed in a growth chamber (L16 : D8, 25.0 ± 1.0°C) (Pavela 2015).