3.7. Stability
Biosensor stability was tested on 0.5mM ethanol, pH 7,
25 ◦C, for 8 days with five determinations per day (Fig. 5).In between, the sensors was kept in buffer solution at 4 ◦C.
After 5 days, the signal has decreased to 70% of its initial
value in the first day, and reached 40% after 8 days.
Ethanol concentration in alcoholic beverages was determined
using the microbial biosensor. Dilutions between 40
and 500 times were performed, depending on the beverage.
The results were compared with the enzymatic spectrophotometric
method.
The correlation coefficient of the experimental data,
0.9972 (Fig. 6) shows a good correlation between the results
obtained by the biosensor and spectrometric methods.
The coefficient of variation was >6% for the biosensor and
>2.5% for the spectrophotometric method, for determinations
carried out on the same day.
3.7. StabilityBiosensor stability was tested on 0.5mM ethanol, pH 7,25 ◦C, for 8 days with five determinations per day (Fig. 5).In between, the sensors was kept in buffer solution at 4 ◦C.After 5 days, the signal has decreased to 70% of its initialvalue in the first day, and reached 40% after 8 days.Ethanol concentration in alcoholic beverages was determinedusing the microbial biosensor. Dilutions between 40and 500 times were performed, depending on the beverage.The results were compared with the enzymatic spectrophotometricmethod.The correlation coefficient of the experimental data,0.9972 (Fig. 6) shows a good correlation between the resultsobtained by the biosensor and spectrometric methods.The coefficient of variation was >6% for the biosensor and>2.5% for the spectrophotometric method, for determinationscarried out on the same day.
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