3. Results
Primary screening test, the qualitative antibacterial assay revealed that out of the nine different extracts, only methanol extract of A. paniculata leaves posses antibacterial activity against S. aureus ATCC 25923. The methanol extracts of leaves from other two plants, A. maculatum and T. cardifolia exhibited no activity against the pathogens tested (Table 1). Further, A. paniculata leaves were extracted using different concentrations of methanol as solvent and were assayed for antibacterial activity. These assays revealed the highest ac- tivity in 75% methanolic extract (Table 2). Moreover, 75% methanolic extract of A. paniculata was tested for zone of inhibition and MIC against Gram positive bacteria,methicillinresistantS.aureusBHU011,E.faecalisBHU 021byagardilutionmethodandforMICagainstM.tuberculosis by Indirect proportion method (IPM). The results of these bacterial bioassays are given in Table 3. Highest zone of in- hibition(26mm)wasobserved againstS.aureusATCC25923at the dose of 3.0 mg. The antibacterial activities of 75% meth- anol extract from A. paniculata leaves were observed only against the S. aureus ATCC 25923. The extract was not found active against Proteus vulgaris and E. coli ATCC 25922. The MIC value of the active extract against the strains showing promising results in qualitative tests were deter- mined for quantitative assessment of antibacterial potential, which also showed a strong antibacterial potential in it. The lowest MIC value observed was 2.0 mg/ml for S. aureus ATCC