After the acclimation period, fish were starved for 24 h and
anesthetized using eugenol (40 mg L−1) (Filiciotto et al., 2012).
Seabass of uniform size (about 250 g) were weighed and ran-
domly distributed to RAS-C and RAS-O3 at an initial density of
25 ± 0.04 kg m−3.