All participants who had normal vision were assessed their autonomic nervous response by ECG measurements, including hear rate [HR], respiratory raet [RR], SpO2 level and skin temperature [ST], and further invited to scale the intensity of their positive feelings by the subjective emotional experience measure after watching netral and comedy video clips. The self-evaluation of participants was confirmed that our comedy video clip used in this study enabled to evoke their subjective happiness,especially amusement with the highest intensity difference between neutral and comedy video cilp.