The total anthocyanin content was determined
applying the Lambert–Beer law, calculated as mg/100 g of DW,
through the formula: A535 dilution factor=E1%
1 cm;535 where A535 is
the absorbance in the diluted sample and E1%
1 cm;535 is the values factor
(98.2) of molar absorptivity for the acid–ethanol solvent and it
refers to the absorption of a mixture of cranberry anthocyanins in
acid–ethanol, measured in a 1 cm-cell at 535 nm, at a concentration
of 1% (w/v).