Current virus detection is generally performed through the use of biological indexing on indicator plants, enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). In general, bio-indexing is time consuming and labor intensive. Although conventional RT-PCR offers greater sensitivity, it is not cost-effective and difficult to scale-up for high throughput samples. Currently, ELISA remains to be one of the best methods for plant virus detection. A sensitive immunocapture Real-time RT-PCR assay combines the advantages from two widely used virus detection methods, ELISA and RT-PCR to achieve timely and sensitive detection of plant viruses with simple sample preparation by immunocapture technology. This technology provides numerous advantages, including improving sensitivity, reducing contamination risks