The morphological identity of all the collected clones was
confirmed by the authors, KSS and KJA, and the clones were registered
as suggested recently (International Steering Committee
on Duckweed Research and Application, 2013; Zhao et al., 2012).
The clones were sterilized using 2.5% sodium hypochlorite solution
for 2–3 min and cultivated under axenic conditions in
Steinberg medium (Naumann et al., 2007). Plants were maintained
under a daily photoperiodic cycle of 14 h white light
(100 mol m−2 s−1)/10 h dark, at 25 ± 1 ◦C.