2.2.3. Lactose estimation
Lactose was estimated according to Lane Eynon method (Lane & Eynon, 1923).
2.2.4. Scanning electron microscopy
The microstructure of lactose–zinc complex was examined by scanning electron microscopy (SEM). Powder samples of lactose–zinc complex was sprayed on double adhesive tape mounted on aluminum stub. Mounted samples were coated with gold (20 nm thickness) on ion coater (IB-3 ion coater, Eiko, Tokyo, Japan) at 6.7–9.3 Pa for 4 min maintaining the ion current at 6 mA. Samples were finally examined by SEM (EVO 18 special edition, Zeiss, Tokyo, Japan) at an acceleration voltage of 15 kV under high vacuum (1.1 × 10−2 Pa) and micrographs were recorded.
2.2.3 2.2.3. Lactose estimation
Lactose was estimated according to Lane Eynon method (Lane & Eynon, 1923).
2.2.4. Scanning electron microscopy
The microstructure of lactose–zinc complex was examined by scanning electron microscopy (SEM). Powder samples of lactose–zinc complex was sprayed on double adhesive tape mounted on aluminum stub. Mounted samples were coated with gold (20 nm thickness) on ion coater (IB-3 ion coater, Eiko, Tokyo, Japan) at 6.7–9.3 Pa for 4 min maintaining the ion current at 6 mA. Samples were finally examined by SEM (EVO 18 special edition, Zeiss, Tokyo, Japan) at an acceleration voltage of 15 kV under high vacuum (1.1 × 10−2 Pa) and micrographs were recorded.
การแปล กรุณารอสักครู่..
