A potential source of discrepancy between our data and those
from other studies is that the C-terminal fusion of the GFP protein
(approximately 27 kDa) may cause mislocalization through steric
hindrance or interruption of critical C-terminal localization/retention
sequences (Supplementary Table S2). For example, the small
GTP-binding protein Ras2 was localized to the nucleus and the
cytoplasm in this study, but it is known to be localized to the plasma
membrane due to modification of its C terminus with palmitoyl and
farnesyl groups25. Proteins localized to the cell wall26 and subsets of
proteins localized to the peroxisome27 and endoplasmic reticulum28
also contain C-terminal targeting signals, and these were often
mislocalized in this study.