The protein assay is based on the nonspecific binding of BPB to proteins. BPB binds to proteins through a combination of electro static and hydrophobic interactions. Under acidic conditions, BPB can interact with BSA to form a stable blue complex.
The protein assay is based on the nonspecific binding of BPB to proteins. BPB binds to proteins through a combination of electro static and hydrophobic interactions. Under acidic conditions, BPB can interact with BSA to form a stable blue complex.