The homogenate was centrifuged at 4000 ×g for 10 min. 1 mL supernatant and 1 mL TCA were added to 2 mL of 20% TBA solution containing 0.6% (w/v) thiobarbituric acid.
Thehomogenate was centrifuged at 4000 ×g for 10 min. 1 mL supernatantand 1 mL TCA were added to 2 mL of 20% TBA solution containing0.6% (w/v) thiobarbituric acid.