After removal of the PM, all rat appeared normal and survived to the termination (Day15). The mean of body weight gain of treated rats with the PM at the dose of 2,000 mg/kg body weight was not statistically difference from those of the control rats (Table 3). No gross pathological lesions in brain, heart, lung,liver, kidney and genitals were observed on necropsy. The result of this study provided the dermal LD50 of the PM in Wistar rats which was more than 2,000 mg/kg body weight