In both trials, conception rates achieved with a half dose of PNA-particle purified spermatozoa matched the conception rate of a non-purified full dose and were significantly higher than that of a non-purified half-dose. Differences in conception rates with nanopurified semen were observed between sires and also comparing cows to heifers in the first trial, suggesting that heifers and some sires may benefit from nanopurification more than others.