The emulsion obtained was freshly prepared before each experiment. Aliquots (2.5 ml) of the
β-carotene/linoleic acid emulsion were transferred to test tubes containing
0.5 ml of each sample of TRMH at different concentrations.
After 1 h incubation at 50 °C, the absorbance of each sample was measured
at 470 nm. BHA was used as positive standard. The control tube
contained no sample. The test was carried out in triplicate.