In 1977, Pussard and Pons [11] identified three distinct groups of Acanthamoeba (1–3), based on microscopic appearance of the encysted organism. It has been shown that groups 2 and particularly 3 are more virulent in human keratitis. Evidence has also been emerging that epithelial infection with acanthamoeba is augmented by bacterial or viral coinfection [12], with contact lenses providing a platform for both organisms to simultaneously set to work on the cornea.