of the ethanol concentrations assayed (4%, 6% and 8%) following a
similar growth pattern. Growth inhibition increased with increasing
ethanol concentrations (Fig. 1 ). Control growth condition (YPD5
without ethanol) showed am
max of 0.54 h
1
for L76 and L78 and that
value was reduced to 0.41 h
1
for L76 and 0.42 h
1
for L78 already
for 4% ethanol. The intermediate ethanol concentration (6%) was
chosen as initial concentration for the adaptive evolution experi-ment, as a good compromise between growth rate and inhibitory
effect, according to the above mentioned criteria.