To allow wound healing, all factors that delay this process need to be treated: revascularization in case of peripheral ischaemia, administration of offloading to remove ulcer pressure, and adequate debridement and antibiotic therapy in case of local infection. Once that standard care is applied, the evaluation of the wound bed in the treatment of chronic DFUs is a key passage. In relation to this consideration, the concept of wound bed preparation which is defined as the systemic approach to study and correct the molecular environment of the wound bed,should be implemented.