The S. cerevisiae strain used in this study was isolated from fementation vats in the state Minas Gerais, Brazil using the modified
method of Oliveira et al. (2008). Samples were collected from fermentation vats in sterile 150-mL flasks. Samples were diluted
1:100,000 in sterile water in triplicate and were inoculated on
YNB medium supplemented with ampicillin at 30 C for 3 days.
The isolates were first classified on the basis of morphological
characteristics and then tested for their ability to grow at different
temperatures (37 and 42 C) in the presence of 10% (w/v) ethanol.
Selected colonies were grown on YP medium containing 2% (w/v)
glucose supplemented with 10% (w/v) ethanol at 37 C