initial denaturationat 94◦C for 5 min followed by 35 cycles of denaturation at 94◦C for1 min, annealing at 55◦C for 1 min, and extension at 72◦C for 1 min,followed by a final extension at 72◦C for 7 min. PCR products were analyzed by 1.5% agarose gel electrophoresis in TBE buffer, pH 8.0