Comparing the three methods one has to consider that the
AOAC and the new iCheck CAROTENE method are very similar with
regard to the quantification of the carotenoids. Both use the
absorption at 450 nm and the extinction coefficient (E1%) of
ß-carotene for the quantification as total carotenoids. But they
are different with regard to the extraction procedure. However,
the HPLC and the iCheck CAROTENE method are similar with
regard to the extraction procedure and solvent composition. Both
extract all carotenoids into hexane as organic solvent. Quantification
was done at the same wavelengths but concentration was calculated using the HPLC method as individual carotenoids based on their individual extinction coefficients.