The 25-min program was the following, 0–5 min,
100% solvent A; 10–11 min, increase from 0% to 100% solvent B;
11–19 min, 100% solvent B; 19–20 min, increase from 0% to 100%
solvent A; and 20–25 min, 100% solvent A. Carotenoid and tocopherol
contents were determined by diode array detection at 445
and 298 nm, respectively.