Ammonia and phosphorus (orthophosphate) determination
were based on Phenate Method and Vanadomolybdophosphoric
Acid Method adapted from the Standard Method for the Examination
ofWater andWastewater (APHA, 2012). Sampled water was
clarified from the MA and EM biomass by centrifugation at
5000 rpm, 15 min to obtain clear supernatant which was subjected
immediately to water quality analysis for the determination of
ammonia and phosphorus. Daily sampling was carried out and the
sample was processed instantaneously to prevent sample degradation
if preserved.