The plasmids, pcDNA3, pcDNA3-IHNV-G, pcDNA3-SHRV-G, and
pcDNA3-SVCV-G, were each used to transform competent Escherichia coli
JM109, and these transformed organisms were grown in the presence of ampicillin.
Large-scale preparations of each plasmid were made with the Qiagen
(Valencia, Calif.) plasmid purification kit, according to the manufacturer’s protocol.
The concentration of purified plasmids was determined by optical density
at 260 nm