Experimental protocol
Subjects visited the laboratory on two separate occasions, with at least 48 h between visits, to complete familiarization trials. Subjects also visited the laboratory on a third occasion, at least 7 days after the last familiarization trial, to complete the experimental protocol. The first two visits included 60-min best effort time trials performed at either a 5% or a 7% constant grade using an adjustable fit bicycle placed on a computrainer indoor trainer as outlined below. Whether a participant cycled at 5% or 7% was determined based on self-reported cycling ability, with the stronger cyclists riding a 7% grade, and the weaker subjects riding a 5% grade. This system of handicapping resulted in a similar total distance completed for all cyclists. (For more on this procedure, see Exercise equipment and performance feedback system below)