On the contrary, the samples collected at the end of the anaerobic (WWanaer) and aerobic (WWfinal) reaction phases of a typical FSBR1 cycle appeared to be potentially cytotoxicity and genotoxic since considerable inhibition of yeast growth (>90% relative to control)and induction of gene expression (1.8 < fold change values < 2.8,depending on the indicator gene) occurred (Fig. 6A and B, respectively). Nevertheless, the toxicity decreased slightly from day 21to day 29; this was mostly evidenced by the decreases in the fold change modifications in the expression of the genotoxicity-indicator genes RAD54 and RNR3 to values close to one (similar to the samples WWfeed or WWcontrol) and in the expression of the general-stress-response gene GRE2 from around 2.8, in average,to