Materials and methods
2.1. Animals
The procedures and protocol design described here were completely
in accordance with the guidelines and regulations of the
Animal Research Center of Mashhad Medical Science. Five healthy
3-year-old male beagle dogs, weighing between 18 and 20 kg were
used in this study. They had intact teeth and the clinical and radiographic
survey showed no previous periodontal disease. Pre-study
preparation included scaling and daily plaque control to obtain
gingival health.