Plant material The experiment was conducted using 2-year-old assam tea (Camellia sinensis var. assamica) plant, which grown from seeds in with commercial soil (pH at 5.5-6) under controlled conditions the plastic-house of the School of Bioresources and Technology, King Mongkutps University of Technology Thonburi (Bangkuntein), Bangkok, Thailand. Using full strength Hewittps nutrient solution will be applied every 7-day and water supply every day.