Aliquots of 0.1 mL of each sample were added to
2.9 mL DPPH (Sigma-Aldrich Co., Milan, Italy) methanol solution
(120 mmol/L). The absorbance was measured at 517 nm after an
incubation step at 30 C for 30 min in the dark. DPPH methanol
solutionwas used as control. The results were calculated as follows:
DPPH radical scavenging activity (%) ¼ 100 e (absorbance of sample/
absorbance of control) 100