2.2. Total polyphenol
The total phenolic content of the extract was determined using
Folin–Ciocalteu reagent (Braca, De Tommasi, Di Bari, 2001). In
Brief, to 3 mL of appropriately diluted extract 0.5 mL of FCR was
added followed by incubation at room temperature (10 min) and
added 2 mL of 7% Na2CO3 solution. The mixture was boiled for
1 min, and absorbance of the colour was recorded at 750 nm in
spectrophotometer (Shimadzu, Kyoto, Japan). The results were
expressed in g gallic acid equivalent (GAE)/g of fruit, on dry weight
basis.