2.7.3. Immunological activity
Lysozyme activity in serum was determined following Parry et al.
(1965). The phagocytic activity assay was determined by adopting a
modified method of Yoshida and Kitao (1991). The phagocytic index
(PI) was determined as: PI = Average number of beads per cell /
Number of phagocytizing cells. The respiratory burst activity of the
peripheral blood leucocytes was determined (Secombes, 1990). The
alternative complement pathway activity (ACH50) was determined
by the following Yanno (1992). The superoxide anion production,
bactericidal activity and serumanti-protease activityweremeasured