Materials of Pikovskaya broth medium are glucose 10 g; Ca3(PO4)2 5 g; (NH4)2 SO4 0,5 g; MgSO.2H2O 0,1 g;MnSO4 25
mg; FeSO425 mg; KCl 0,2 g, yeast extract 0,5 g; and agar 15 g, dissolved in sterile water until
volume of 1 l. Pipette 30 ml of the suspension and put in the Erlenmeyer, containt Pikovskaya
broth medium, and incubated in rotary shaker at 150 rpm for 7 days.