Size exclusion chromatograms of hydrolysates at different DHs are shown in Fig. 3. Samples were
analysed after a 4-h hydrolysis. From the chromatographic data, we observed that all hydrolysates
were composed of low molecular weight peptides . Hydrolysates with a 11.1% DH was characterized by
a low percentage of peptides with molecular weight above 5000. A regular decrease of this fraction was
observed as the enzyme/substrate ratio increased(Table 1). The enzymatic treatment by Umamizyme
of the tuna stomach proteins yielded a high quantity of small peptides below 500 Da ranging from 39%
(11.1% DH) to 58% (22.5% DH).