Corning Cellgro) and RPMI 1640 (112-025-101, Quality Biological) respectively, supplemented with 10% FBS (16140071, Invitrogen) and Penn-Strep (120-095-721, Quality Biological). Cells harvested using 0.25% Trypsin EDTA (25300–054, Gibco), within passages 2–8, for seeding the culture device and PET transwell controls.