using 30 ml of 0.05 M HCl at 90 C for 60 min with constant stirring (50 rpm).
The extracts were then cooled to room temperature (w30 C) and centrifuged at 2700g for 15 min.
The supernatants were collected and dispersed in an equal volume of 2-propanol.
Pectin was precipitated by adjusting the pH of the dispersion medium to 3.5 and was allowed to stand for 6 h.
The precipitate was collected by centrifugation, dispersed in 2-propanol (25 ml), stirred for 1 h, and centrifuged again at 2700g for 15 min.
These steps (dispersing and centrifuging) were repeated twice.
The precipitate was then dried at 60 C for 5 h.