All media were supplemented with sucrose (30 g l1) and gelled
with agar (6 g l1). The pH of the medium is adjusted to 5.8 before
gelled with agar and sterilized.
All cultures were incubated at 23 1 8C with 16 h light (at an
irradiance of 36 mmol m2 s1).
The plantlets were transferred to greenhouse in pots containing
peat enriched with mineral manure (Klansman Protagrond H).