Human monocytic (THP-1) cell line was obtained from National
Centre for Cell Sciences, Pune, India. The cells were cultured at
37 C in 5% CO in RPMI media supplemented with 10% fetal
bovine serum (FBS) and 100 mg/mL pen-strep. Thereafter the 2
experiments were conducted at a cell density of 5 10 cells/mL.
An acute inflammatory stimulus was given to the cells by incubating
them with 0.5 mg/mL of LPS for 3 h at 37 C in 5% CO
(Kokkiripati et al., 2011). In order to stimulate the differentiation
of THP-1 cells to macrophages, the cells were treated with 5 ng/mL
of PMA for 48 h at 37 C in 5% CO2
as described in Tsuchiya et al.
(1982). The images of the cells were taken under an inverted
microscope