Yeast-like cells
used to generate thermal destruction data were prepared by streaking M. circinelloides onto acidified PDA. Plates were incubated at 25 °C for at least 48 h in a GasPack anaerobic jar (Becton,Dickinson and Co., Franklin Lakes, NJ). Typical colony and cellular morphology was observed following incubation. Following extraction from
GasPack anaerobic jars, yeast-like cells were immediately used for thermal destruction experiments.