The story of what happened later that night has been told previously (Cooper, 1997), but I will recount it from my perspective. I couldn’t sleep as I mulled over Dave’s comment while staring at the ceiling in the dark hotel room. I was thinking about the larger cells stuck in the filter stack growing and dividing while the medium passed through for those short few minutes. Then for reasons unknown, since I know nothing about poultry farming, I began visualizing chickens attached all over the ceiling of the room and thinking that the only (living) objects that would fall from the surface would be eggs. That was it. I thought that if cells became stuck within the filter stack while culture medium passed through, the only cells that could be released from the stack would be newborn cells originating from the portion of the adhered cells that was not involved in their adherence I realized that many of the dividing rod-shaped cells might not release progeny at all, or that some newborn cells might reattach, but in the ideal case in which attachment was permanent, only new daughter cells would be released, no matter how few. Experimentally, strong attachment and good flushing with culture medium ought to yield a highly pure population of newborn cells. The best part was that this could be a truly minimally disturbed synchronous population because the process of preparing the synchronous cells simply involved collecting cells falling from a surface-bound culture growing under undisturbed conditions.
The story of what happened later that night has been told previously (Cooper, 1997), but I will recount it from my perspective. I couldn’t sleep as I mulled over Dave’s comment while staring at the ceiling in the dark hotel room. I was thinking about the larger cells stuck in the filter stack growing and dividing while the medium passed through for those short few minutes. Then for reasons unknown, since I know nothing about poultry farming, I began visualizing chickens attached all over the ceiling of the room and thinking that the only (living) objects that would fall from the surface would be eggs. That was it. I thought that if cells became stuck within the filter stack while culture medium passed through, the only cells that could be released from the stack would be newborn cells originating from the portion of the adhered cells that was not involved in their adherence I realized that many of the dividing rod-shaped cells might not release progeny at all, or that some newborn cells might reattach, but in the ideal case in which attachment was permanent, only new daughter cells would be released, no matter how few. Experimentally, strong attachment and good flushing with culture medium ought to yield a highly pure population of newborn cells. The best part was that this could be a truly minimally disturbed synchronous population because the process of preparing the synchronous cells simply involved collecting cells falling from a surface-bound culture growing under undisturbed conditions.
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