Both enzymes were produced by Aspergillus fumigatus KIBGE-IB33.
**Cassava starch.
A large number of microbes including bacteria, yeast and fungi are capable of producing ethanol from fermented sugars [48]. Among them S. cerevisiae is widely used for production of bioethanol because it is not only able to produce high amount of ethanol but it can also tolerate and survive higher concentrations of ethanol formed in the medium [49, 50]. However, most of the yeast strains are not capable to directly ferment complex starchy materials [48]. Therefore, in the current study S. cerevisiae was used for the production of ethanol from glucose which was earlier formed by the action of fungal amylolytic enzymes. Table 4 summarizes the results of bioethanol production after 24 and 48 hours of yeast fermentation and it was noticed that the maximum percent yield of bioethanol (84.0%) was obtained after 48 hours. After 48 hours, the fermented medium was distilled and the percent yield became 53.0%. The concentration and purity of the distilled ethanol was also analyzed using gas chromatography (GC).