2.3. Antimicrobial test activity
The antimicrobial activities of the nanoparticles were confirmed
via the standard Kirby–Bauer disc diffusion method against Staphylococcus
aureus and Escherichia coli. The organisms (106 CFU) were
dispersed on Mueller-Hinton agar, and 10-mm paper discs containing
5 μg of nanoparticles were placed on the medium and were
incubated at 35 1C for 24 h.