Three types of medium a required to induce contains auxin; in suspension. The first m solidified by gar and of em a this dedifferentiates the somatic tissue and provokes initiation and bryogeniccells (primary culture). Thesecond medium, which is liquid me- also contains auxin,ensures themultiplication of these cells. The third dium, which is also liquid but without auxin, allows the cells to express their embryogenic potential. the tissue Culture in a liquid medium is not easy to carry out. When is inoculated, it sinks and rapidly dies because of lack of oxygen. To pre- vent asphyxia, the liquid must be agitated to dissolve air in the medium. The medium, however, has numer- culture of somatic embryos in a liquid ous advantages. The swirling medium naturally separates the embryo which easily observed. Thus, embryos can be fractionated ac- are then the cording to their stages. They can be obtained in great quantity and used as a basis for a large-scale micropropagation.