the 2nd-order derivative spectrophotometric method developed by Lidija et. al. was validated and employed to 5 wild fruits including Ma-kwen Pa (Flacourtia indica), Ma-koh (Schleichero oleosa), Mag Puan (Pouteria campechina) Mag Kee noo (Docynia indica) and Ma-kom (Microcos paniculota) samples without using any pre-separation or background correction techniques, some modifications