Two replications of experiment were conducted to enumerate
the populations of viable Salmonella in inoculated sand during
storage. Triplicate sand samples were assayed for Salmonella at each
sampling points. The inoculated sand, as a dry inoculum medium,
was mixed with the chicken base paste or powder formulations.
The effect of salt levels, specifically, regular salt, 45% reduced-salt
and 90% reduced-salt, on the survival of Salmonella in the chicken
base formulations were evaluated in a single trial. Triplicate samples
for each treatment were analyzed at each sampling interval.
Microbiological counts were transferred into log values for analysis.
Data points are expressed as mean ± standard deviation. A repeated
measure design was used. Analysis of variance for repeated measures
was performed using the MIXED procedure of SAS (version
9.1; SAS Institute Inc., Cary, NC, USA). The covariance structure used
was based on goodness of fit as indicated by Akaike's information
criterion. Means were separated using Tukey's method at a 5% level
of significance.