The extract was filtered through Whatman filter paper #42, and the absorbance was read at 640 nm against the acid acetone blank. Total pigments, as acid hematin, were calculated using the formula:
Total pigment(ppm)=A640×680Total pigment(ppm)=A640×680
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and heme iron was calculated as follows:
Heme iron(ppm)=total pigment(ppm)×8.82/100Heme iron(ppm)=total pigment(ppm)×8.82/100
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