Chokanan mango juice samples subjected to UV-C expo- sure for 15 and 30 min exhibited significant improvement in antioxidant activities and extractability of carotenoids, phe- nolic compounds, and flavonoids, when compared to freshly squeezed juice. In addition, UV-C treatment showed signif- icant reduction in microbial count. The shelf life of UV-C treated juice stored at 4 ± 1 ◦ C was extended for at least 4 weeks longer than freshly squeezed juice. Thermal treat- ment was more effective in completely inactivating microbial growth in the juice, but detrimental effects on quality were observed. Hence, UV-C treatment with appropriate optimiza- tion of processing variables is a feasible alternative to thermal pasteurization. Further research work is needed for combining UV-C with other non-thermal food processing technologies, such as pulsed electric field and high pressure to guaran- tee improved quality juice together with safety standards. Consequently, this study provides more attention for positive implementation of UV-C treatment on a pilot scale.