Isolation and characterization of Actinomycetes
Actinomycetes isolation was done followed by serial dilution on their respective isolation medium. Isolates were purified on actinomycetes isolation agar and ken knight agar medium using streak and counter streak method. Purified actinomycetes isolates were stored at 4°C for further examination. Primary screening was done by morphological behaviour of bacterial colonies such as colony color, size, shape and growth on medium.